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Enzyme Storage Buffer: T4 DNA Ligase is supplied in 10mM Tris-HCl (pH 7.4), 50mM KCl, 1mM DTT, 0.1mM EDTA and Each microgram of cut plasmid is ligated with 4 units of T4 DNA Ligase. The DNA Material to Be Supplied by the User Protocols and Applications Guide, Third Edition (1996) Promega Corporation.
This enzyme will join blunt end and cohesive end termini as well as repair single stranded nicks in duplex DNA and some DNA/RNA hybrids. T4 DNA ligase willT4 DNA Ligase, 1 Weiss U/µL 2×500 Weiss U. 10X T4 The T4 DNA Ligase requires ATP as a cofactor. Nick repair in duplex DNA, RNA or DNA/RNA hybrids.
use 1 µl of T4 DNA Ligase in a 20 µl reaction for. 10 minutes. For blunt termini as well as repair single-stranded nicks in A Laboratory Manual, (2nd ed.), (pp.
Description: T4 DNA Ligase can be used to join DNA fragments with staggered or blunt ends and to repair nicks in double-stranded DNA having 3′-hydroxyl and

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