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RNA extraction and cDNA synthesis. Each sample was treated with DNaseI (Invitrogen) according to the instruction manual to remove hypothetically contaminating DNA. (cDNA) (doublestrand DNA version of an mRNA molecule) with the iScriptTM cDNA Synthesis Kit (BIORAD) following the
gram cDNA was synthesized for each brain sample using the iScriptTM cDNA Synthesis Kit (BIO-RAD, Hercules, CA). 2.4.2. Endogenous Control Gene and the Analysis Method. We considered both the Relative Standard Curve and the ??Ctmethodsforthereal-timePCRquanti?cationofSNCA
• Efficiencies of cDNA synthesis measured in two-step qRT-PCR • Choosing the best qRT-PCR product for your application •O ne step vs two step • Summary. 3 – High performance and complete kit for 2-step real-time RT-PCR, for
The source of RNA was skin fibroblasts extracted using QIAamp RNA Mini Kit (Qiagen Inc, Germantown, Maryland, USA) and DNase treated by the RNase-Free DNase Set (Qiagen), according to the manufacturer’s recommendations and used for cDNA synthesis using iScriptTM cDNA synthesis kit and Poly T oligonucleotide primers (Applied Biosystems, Carlsbad
WizScript™ cDNA Synthesis Kit is a complete system for the efficient synthesis of first strand cDNA from RNA templates. The recombinant Ribonuclease Inhibitor, supplied with the kit effectively protects RNA template from degradation. The kit is also supplied with both oligo dT and random primers.
Application The Transcriptor First Strand cDNA Synthesis Kit is designed to reverse tran-scribe RNA (mRNA, total RNA, viral RNA, and in vitro transcribed RNA) from a Ordering Information or to our Special Interest Site on manual nucleic
ZAP Express cDNA Synthesis Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit Instruction Manual Catalog #200403 (ZAP Express cDNA Synthesis Kit) and #200451 (ZAP Express cDNA Gigapack III Gold Cloning Kit) Caution DO NOT substitute the components in this kit with components from another kit.
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