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http://www.amazon.com The EST2ncRNA pipeline (see Figure 1) was used to analyse the PigEST data.In the following we present our results in terms of the number of conreads that contain at least one high confidence RNA structure (RNAz classificator p > 0.9 and thermodynamical stability score z < -3), because only a small fraction of conreads contains two or more predicted structured loci.
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The Saccharomyces cerevisiae genome has undergone extensive intron loss during its evolutionary history. It has been suggested that the few remaining introns (in only 5% of protein-coding genes) are retained because of their impact on function under stress conditions. Here, we explore the possibility that novel noncoding RNA structures (ncRNAs) are embedded within intronic sequences and are
index (SCI=EA/E).Based on Z-score and SCI, RNAz calculates a combined score, the so-called “RNA class probability” which also is referred to as “P-value”.If p>0.5, RNAz classifies an alignment as “RNA”, meaning that RNAz has detected an unusually stable and/or unusually
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