To understand real-time PCR it is easier to begin with the principles of a basic PCR: PCR is a technique for amplifying DNA. There are 2 reasons why you may
6 Sep 2018 geNorm: Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple The manual is available here.An exhaustive guide to assay design for quantitative real-time PCR. The book describes the basic concepts important for amplicon selection and primer and
The Quantitative PCR Technical Guide from Sigma-Aldrich is intended to Real Time quantitative PCR (qPCR) is very similar to traditional. PCR. The major
The polymerase chain reaction (PCR) is one of the most powerful technologies in molecular biology. Using PCR, specific sequences within a DNA or cDNA